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首頁 > 技術(shù)文章 > OriGen PermaLife 細(xì)胞培養(yǎng)袋凍存袋

OriGen PermaLife 細(xì)胞培養(yǎng)袋凍存袋

更新時(shí)間:2019-03-06瀏覽:2939次

OriGenPermaLife Cell Culture Bags

OriGen PermaLife Bags are used for cell storage, cryopreservation, genetic engineering, and vector production.

  • Cell Culture: The OriGen PermaLife™ bag represents the optimal way to harvest, expand, protect and preserve critical cell cultures. The bags have received marketing clearance from the FDA and areindicated for use in protecting, storing and freezing cells and tissues. Made compley of inert FEP, the PermaLife bag contains no plasticizers or chemical residuals to leach into or alter delicate cell cultures.
  • Freezing Bags: The PermaLife storage bags can also be used as freezing bags for cell suspensions. FEP remains flexible at liquid nitrogen temperatures, and is unsurpassed as a cryogenic container.

Features of the OriGen PermaLife Cell Storage Bags

  • Inert: FEP is chemically and biologically inert, so the bag will not alter the culture or the medium. FEP neither combines with any element, nor gives up any components of its own to the solution held in the cell culture bag.
  • Breathable: FEP film is gas permeable and gas transfer rates for oxygen, carbon dioxide and nitrogen have been published. However, FEP is practically impermeable to water so water losses from the cell culture is negligible. Humidified incubators - potential sources of mold and contamination - are not necessary.
  • Sealed: The PermaLife cell storage bag is a closed system, so the possibility of contamination is greatly reduced.
  • Optical: The PermaLife bag has an optical transmission of greater than 95%. Observation of cell cultures under a microscope is easy, and can be done without risking contamination of the sample.
  • Thermal: The PermaLife bag can be used as a freezing bag, and remains flexible with no significant decrease in mechanical strength at temperatures from cryogenic storage (-196ºC) to high autoclave (+137ºC).
  • Safety Features: Each bag has a hanger hole.  Each bag comes with a needle-free adapter to reduce the chance of needle sticks.
  • Needle-Free: A sterile, needle-free, luer-actuated adapter is attached to each bag. This self-sealing valve makes it easy to feed and harvest sensitive cells without having to expose them to contamination. The valve face is swabable and can be activated with any male luer device, such as a standard syringe.

OriGen PermaLife Technical Information

Catalog Number

Volume* Nominal

Volume* Maximum

Width (cm)

Length (cm)

Surface Area, cm²

 

CO² 

 

H2O‡‡

 Stock/Custom

PL07-2G

7

12

3

6.3

38

44

98

19

0.03

Stock   

PL30-2G

30

50

8.9

6.1

108

126

281

54

0.08

Stock

PL70-2G

70

145

8.9

9.7

172

199

444

86

0.12

Stock

PL120-2G

120

265

8.9

14

248

288

643

124

0.17

Stock

PL240-2G

240

725

14

19.3

540

627

1400

270

0.38

Stock

PL325-2G

325

1100

14

25.9

725

842

1879

363

0.51

Stock

PL300-2G

300 

1000 

14

23.37

654

759

1695

327 

0.46 

Custom 

PL500P-2G

500 

1500

29.21 

18 

1052

1220

2724 

526 

0.74

Custom

PL750-2G

750

1500

29.21

26.5

1548 

1796

4010

774

1.08 

Custom 

PL1000P-2G

1000 

3000

29.21

35

2045

2372

5296 

1022

 1.43

 Custom

PL2000P-2G

2000

3000 

47

45 

4230

4907 

 10956

2115 

 2.96

 Custom

OriGen PermaLife

Table Legend:
Length and Width in Table are dimensions of the active cell culture compartment only.
* Nominal volume in ml: Freezing volume of bag at 1 cm thickness
Total surface area, both sides of bag
‡Gas transfer is in ml/ 24 hours at 25°C (Ref: "Teflon FEP Film" DuPont Bulletin E-80412)
‡‡Water transmission is in g per 24 hours at 40°C (Ref: "Teflon FEP Film" DuPont Bulletin E-80412) 

Note: theOriGen PL07 has only one port and is sold with one needle-free valve 

"P" in the catalog number denotes it includes a PVC spike port 

 

OriGen PermaLifeOriGen 細(xì)胞培養(yǎng)袋——多國認(rèn)證
OriGen生物醫(yī)學(xué)公司具有ISO 13485認(rèn)證,其全部產(chǎn)品都具有美國FDA上市許可,并獲得歐盟CE認(rèn)證,部分產(chǎn)品已完成中國藥監(jiān)局注冊(cè)(獲第三類醫(yī)療器械證)。

Custom Configurations
Custom configurations and more are available.

OriGen Evolve 細(xì)胞培養(yǎng)袋
比OriGen PermaLife 細(xì)胞培養(yǎng)袋更經(jīng)濟(jì)實(shí)惠,支持30-40℃下細(xì)胞培養(yǎng),適用于淋巴細(xì)胞、NK細(xì)胞、雜交瘤細(xì)胞、CAR-T細(xì)胞的大規(guī)模培養(yǎng)。

 

OriGen 細(xì)胞培養(yǎng)袋新品到貨

OriGen現(xiàn)貨訂購

 

Origen公司簡介

Origen(奧利金)生物醫(yī)學(xué)公司成立于1990年,是一家總部設(shè)在美國德克薩斯州*市的醫(yī)療器材制造公司。OriGen Biomedical奧利金生物醫(yī)學(xué)公司為細(xì)胞培養(yǎng)和冷凍保存提供關(guān)鍵性的器材。制造的產(chǎn)品非常適用于組織存儲(chǔ),細(xì)胞培養(yǎng)和存儲(chǔ),干細(xì)胞擴(kuò)增和長期冷凍保存,并且特別適合于液氮冷凍保存。

 

OriGen Biomedical奧利金生物醫(yī)學(xué)公司具有ISO13485認(rèn)證。我產(chǎn)品都具有美國國家食品藥品管理局FDA的上市許可證,在加拿大通過了注冊(cè),并已獲得歐盟CE認(rèn)證。

 

OriGen Biomedical奧利金生物醫(yī)學(xué)公司的產(chǎn)品被世界各地的用戶廣泛地采用,也在多個(gè)國家和地區(qū)通過了注冊(cè)。十幾年來,OriGen Biomedical奧利金生物醫(yī)學(xué)公司一直竭誠地為中國用戶提供的產(chǎn)品和服務(wù)。伴隨著中國干細(xì)胞存儲(chǔ)和應(yīng)用的發(fā)展,OriGen Biomedical奧利金生物醫(yī)學(xué)公司的產(chǎn)品在中國的臍血庫,生物技術(shù)公司,科研單位,醫(yī)療機(jī)構(gòu)等領(lǐng)域越來越多的用戶使用。

 

OriGen Biomedical奧利金生物醫(yī)學(xué)公司提供的產(chǎn)品和客戶服務(wù)。我們傾聽客戶的意見,并與他們合作,開發(fā)出滿足其需求的產(chǎn)品。我們致力于為客戶提供量身定制的產(chǎn)品以滿足他們的特定需求。

 

PermaLife Cell Culture Bags 英文介紹

產(chǎn)品用途:

 

Cell Culture and Cell Expansion

Cord Blood Banking

Genetic Engineering

Vector Production

Cryopreservation

 

產(chǎn)品優(yōu)勢(shì):Advantages of the OriGen PermaLife Cell Culture Bags

The OriGen PermaLife  Cell Culture bags represent the optimal way to harvest, expand, concentrate, and freeze critical cell cultures. Made of inert FEP, the PermaLife  bag contains no plasticizers or chemical residuals which

could leach into and alter delicate cell cultures. Because FEP is inert, antibodies, cytokines, and other genetic material are unaected by the PermaLife  bag.

PermaLife  FEP bags are a closed system, so the possibility of contamination is greatly reduced. Bags are sold sterile. PermaLife bags are gas permeable, and provide a high rate of oxygen transfer to cells under culture.

Additionally, water permeability is very low, so water loss is negligible and humidied incubators are not required.

 

The PermaLife  bag has an optical transmission of greater than 95%. Observation of the cultures under a microscope is possible without risking contamination of the culture.

It is also ideal for cryogenic preservation. The PermaLife  bag remains exible from -200°C to +200°C. There is no signicant decrease in mechanical strength at temperatures from cryogenic storage (-196°C) to high autoclave (+137°C).

 

Swabbable, needle-free valves are provided on every bag to preserve sterile access and reduce the chance of needle sticks. PermaLife bags are sold with one female luer and one needle-free valve. The PL07 only has one port and is sold with one needle-free valve. PermaLife  Catalog numbers including a “P” sux are sold with a needle-free valve and a PVC spike port.